Kenneth Goldie

Research

Broadly based on the Structural analysis of proteins and macro molecular complexes by transmission electron microscopy (TEM) to gain insights into biological processes, function and their relation to disease.

    Short Biography

    Ken Goldie from New Zealand holding dual Swiss Nationality is a research associate and the Facility Manager in the Stahlberg group, C-CINA of the Biozentrum since 2009. Has worked in the Electron Microscopy field since 1980, specialising in high-resolution transmission electron microscopy and scanning EM. Attained a PhD in Structural Biology from The University of Auckland New Zealand and has held positions in 3 New Zealand Universities, Cancer Research UK London, EMBL Heidelberg, and the University of Melbourne Australia. Responsible for the day-to-day running of the microscope facilities in C-CINA and the instruction of new researchers and lab visitors

    Publications

    -+

    Cryo-EM structures of the por....

    Piper, S., Brillault, L., Rothnagel, R., Croll, T., Box, J., Chassagnon, I., Scherer, S., Goldie, K., Jones, S., Schepers, F., Hartley-Tassell, L., Ve, T., Busby, J., Dalziel, J., Lott, S., Hankamer, B., Stahlberg, H., Hurst, M., Landsberg, M

    (submitted), (2019)

    83

    Deuterium induces a distinctive Escherichia coli proteome that correlates with the reduction in growth rate.

    Opitz, C., Ahrne, E., Goldie, K., Schmidt, A., Grzesiek, S

    J. Biol. Chem. (in press), (2019)

    PubMed

    DOI: DOI

    82

    Rapid Structure Determination of Microcrystalline Molecular Compounds Using Electron Diffraction.

    Gruene, T., Wennmacher, JTC, Zaubitzer, C, Holstein, JJ, Heidler, J, Fecteau-LeFebvre, A., De Carlo, S, Mueller, E, Goldie, K., Regeni, I, Li, T, Santiso-Quinones, G, Steinfeld, G, Handschin, S, van Genderen, E., van Bokhoven, JA, Clever, GH, Pantelic, R

    Angew. Chem. Int. Ed. Engl. 57(50), 16313-16317 (2018)

    PubMed

    DOI: DOI

    81

    Lewy pathology in Parkinson's disease consists of a crowded organellar membranous medley

    Shahmoradian, S., Lewis, A., Genoud, C., Graff, A., Pérez Navarro, P., Goldie, K., Sütterlin, R., Castano-Diez, D., Ingrassia, A., Rozemuller, A.J.M., Paepe, A.D., Erny, J.Staempfli, A., Hoernschemeyer, J., Niedieker, D., El-Mashtoly, S.F., Gerwert, K., Bohrmann, B., Britschgi, M., Stahlberg, H., van de Berg, W.D.J., Lauer, M.E.

    (submitted), (2019)

    80

    Phospholipid-block copolymer hybrid vesicles with lysosomal escape ability

    Zong, W., Thingholm, B., Itel, F., Schattling, P.S., Brodszkij, E., Mayer, D., Stenger, S., Goldie, K., Han, X., Städler, B.

    Langmuir 34(23), 6874-6886 (2018)

    PubMed

    DOI: DOI

    79

    3D correlative electron microscopy reveals continuity of Brucella-containing vacuoles with the endoplasmic reticulum

    Sedzicki, J., Tschon, T., Huey-Low, S., Willemart, K., Goldie, K., Lewtesson, J.-J., Stahlberg, H., Dehio, C.

    J. of Cell Science 131(4), (2018)

    PubMed

    DOI: doi

    77

    Cryo-EM reconstruction of Type VI secretion system baseplate and sheath distal end

    Nazarov, S., Schneider, J.PO., Brackmann, M., Goldie, K., Stahlberg, H., Basler, M.

    EMBO Journal 37, e97103 (2018)

    PubMed

    DOI: DOI

    76

    CryoEM structure of the extended type VI secretion system sheath–tube complex

    Wang, J., Brackmann, M., Castano-Diez, D., Kudryashev, M., Goldie, K., Maier, T., Stahlberg, H., Basler, M.

    Nature Microbiology 2(11), 1507-1512 (2017)

    PubMed

    DOI: DOI

    74

    Atomic structure of granulin determined from native nanocrystalline granulovirus using an X-ray free-electron laser

    Gati, C., Oberthuer, D., Yefanov, O., Bunker, R.D., Stellato, F., Chiu, E., Yeh, S.M., Aquila, A., Basu, S., Bean, R., Beyerlein, K.R., Botha, S., Boutet, S., DePonte, D.P., Doak, R.B., Fromme, R., ..., Goldie, K., ..., Barty, A., Chapman, H.N.

    Proc Natl Acad Sci U S A 114(9), 2247-2252 (2017)

    PubMed

    DOI:

    73

    Cell-free reconstitution reveals centriole cartwheel assembly mechanisms

    Guichard, P., Hamel, V., Le Quennec, M., Banterle, N., Iacovache, I., Nemcikova, V., Flückiger, I., Goldie, K., Stahlberg, H., Levy, D., Zuber, B., Gönczy, P.

    Nature Communications 8:14813, (2017)

    PubMed

    DOI:

    71

    Structure of the Full-length VEGFR-1 Extracellular Domain in Complex with VEGF-A

    Markovic-Mueller, S, Stuttfeld, E., Asthana, M., Weiner, T., Bliven, S., Goldie, K., Kisko, K., Capitani, G., Ballmer-Hofer, K.

    Structure 25(2), 341-352 (2017)

    DOI: 10.1016/j.str.2016.12.012

    70

    Cullin-RING ubiquitin E3 ligase regulation by the COP9 signalosome

    Cavadini, S., Fischer, E.S., Goldie, K., Boehm, K., Lingaraju, G.M., Jones, M., Bunker, R.D., Richkule, R., Pantelic, R., Mohamed, W.I., Hassiepen, U., Beckwith, R.E.J., Stahlberg, H., and Thomae, N.H.

    Nature 531(7596), 598-603 (2016)

    PubMed

    DOI: 10.1038/nature17416

    68

    Atomic structure of bacteriophage T4 baseplate and its function in triggering sheath contraction

    Taylor, N., Prokhorov, N.S., Guerrero-Ferreira, R.C., Shneider, M.M., Browning, C., Goldie, K., Stahlberg, H., Leiman, P.G.

    Nature 533(7603), 346-352 (2016)

    PubMed

    DOI: 10.1038/nature17971

    67

    Phospholipid-polymer amphiphile hybrid assemblies and their interaction with macrophages

    Panneerselvam, K., Lynge, M. E., Riber, C. F., Mena-Hernando, S., Smith, A. A., Goldie, K., Zelikin, A. N., Stadler, B.

    Biomicrofluidics 9(5), 52610 (2015)

    PubMed

    66

    openBEB: open biological experiment browser for correlative measurements

    Ramakrishnan, C., Bieri, A., Sauter, N., Roizard, S., Ringler, P., Müller, S., Goldie, K., Enimanev, K., Stahlberg, H., Rinn, B., Braun, T.

    BMC Bioinformatics 15, 84 (2014)

    PubMed

    65

    Poly(N-isopropylacrylamide)/poly(dopamine) capsules

    Zhang, Y., Teo, B. M., Goldie, K., Stadler, B.

    Langmuir 30(19), 5592-8 (2014)

    PubMed

    64

    Carbon nanotube growth on AlN support: comparison between Ni and Fe chemical states and morphology

    Eren, B., Marot, L., Steiner, R., de los Arcos, T., Duggelin, M., Mathys, D., Goldie, K., Olivieri, V., Meyer, E.

    Chem Phys Lett 609, 82-87 (2014)

    DOI: DOI

    63

    Confined multiple enzymatic (cascade) reactions within poly(dopamine)-based capsosomes

    Hosta-Rigau, L., York-Duran, M. J., Zhang, Y., Goldie, K., Stadler, B.

    ACS Appl Mater Interfaces 6(15), 12771-9 (2014)

    PubMed

    62

    Cryo-electron microscopy of membrane proteins

    Goldie, K., Abeyrathne, P., Kebbel, F., Chami, M., Ringler, P., Stahlberg, H.

    Methods Mol Biol 1117, 325-41 (2014)

    PubMed

    61

    Synthesis of anisotropic, amphiphilic grafted multi-star polymers and investigation of their self-assembling characteristics

    Blencowe, A., Tan, J. F., Goh, T. K., Goldie, K., Zhang, X. H., Qiao, G. G.

    Aust J Chem 67(1), 49-58 (2014)

    DOI: DOI

    60

    Development of amphiphilic multi-star polymers with highly grafted pyrene connectors as unimolecular encapsulation devices

    Blencowe, A., Tan, J. F., Goh, T. K., Goldie, K., Qiao, G. G.

    Polymer Chemistry 5(5), 1682-1692 (2014)

    DOI: DOI

    59

    Poly(vinyl alcohol) physical hydrogel nanoparticles, not polymer solutions, exert inhibition of nitric oxide synthesis in cultured macrophages

    Andreasen, S. O., Chong, S. F., Wohl, B. M., Goldie, K., Zelikin, A. N.

    Biomacromolecules 14(5), 1687-95 (2013)

    PubMed

    58

    Structural and mechanistic insights into VEGF receptor 3 ligand binding and activation

    Leppanen, V. M., Tvorogov, D., Kisko, K., Prota, A. E., Jeltsch, M., Anisimov, A., Markovic-Müller, S., Stuttfeld, E., Goldie, K., Ballmer-Hofer, K., Alitalo, K.

    Proc Natl Acad Sci U S A 110(32), 12960-5 (2013)

    PubMed

    57

    Electron microscopy analysis of 2D crystals of membrane proteins

    Abeyrathne, P.D., Arheit, M., Kebbel, F., Castano-Diez, D., Goldie, K., Chami, M., Renault, L., Kühlbrandt, W., Stahlberg, H.

    Comprehensive Biophysics 1, 277-310 (2012)

    DOI: 10.1016/B978-0-12-374920-8.00119-3

    56

    Myoblast cell interaction with polydopamine coated liposomes

    van der Westen, R., Hosta-Rigau, L., Sutherland, D. S., Goldie, K., Albericio, F., Postma, A., Stadler, B.

    Biointerphases 7(42008), 8 (2012)

    PubMed

    55

    Noncore residues influence the kinetics of functional TTR(105-115)-based amyloid fibril assembly

    Bongiovanni, M. N., Puri, D., Goldie, K., Gras, S. L.

    J Mol Biol 421(3), 256-69 (2012)

    PubMed

    54

    Assembly of poly(dopamine) films mixed with a nonionic polymer

    Zhang, Y., Thingholm, B., Goldie, K., Ogaki, R., Stadler, B.

    Langmuir 28(51), 17585-92 (2012)

    PubMed

    53

    Connecting micro-fluidics to electron microscopy

    Kemmerling, Simon, Ziegler, J., Schweighauser, G., Arnold, S., Giss, D., Müller, S., Ringler, P., Goldie, K., Goedecke, N., Hierlemann, A., Stahlberg, H., Engel, A., Braun, T.

    J Struct Biol 177(1), 128-134 (2012)

    PubMed

    52

    Surface-adhered composite poly(vinyl alcohol) physical hydrogels: polymersome-aided delivery of therapeutic small molecules

    Hosta-Rigau, L., Jensen, B. E., Fjeldso, K. S., Postma, A., Li, G., Goldie, K., Albericio, F., Zelikin, A. N., Stadler, B.

    Adv Healthc Mater 1(6), 791-5 (2012)

    PubMed

    51

    The outer membrane protein LptO is essential for the O-deacylation of LPS and the co-ordinated secretion and attachment of A-LPS and CTD proteins in Porphyromonas gingivalis

    Chen, Y. Y., Peng, B., Yang, Q., Glew, M. D., Veith, P. D., Cross, K. J., Goldie, K., Chen, D., O'Brien-Simpson, N., Dashper, S. G., Reynolds, E. C.

    Mol Microbiol 79(5), 1380-401 (2011)

    PubMed

    50

    Transmission electron microscopy of amyloid fibrils

    Gras, S. L., Waddington, L. J., Goldie, K.

    Methods Mol Biol 752, 197-214 (2011)

    PubMed

    49

    Ultrastructure of the asexual blood stages of Plasmodium falciparum

    Hanssen, E., Goldie, K., Tilley, L.

    Method Cell Biol 96, 93-116 (2010)

    PubMed

    47

    Aromatic residues in the C-terminal helix of human apoC-I mediate phospholipid interactions and particle morphology

    James, P. F., Dogovski, C., Dobson, R. C., Bailey, M. F., Goldie, K., Karas, J. A., Scanlon, D. B., O'Hair, R. A., Perugini, M. A.

    J Lipid Res 50(7), 1384-94 (2009)

    PubMed

    46

    Capsosomes: subcompartmentalizing polyelectrolyte capsules using liposomes

    Stadler, B., Chandrawati, R., Goldie, K., Caruso, F.

    Langmuir 25(12), 6725-32 (2009)

    PubMed

    45

    Unravelling the structure of the lamellipodium

    Small, J. V., Auinger, S., Nemethova, M., Koestler, S., Goldie, K., Hoenger, A., Resch, G. P.

    J Microsc 231(3), 479-85 (2008)

    PubMed

    44

    Structural analysis of vimentin and keratin intermediate filaments by cryo-electron tomography

    Norlen, L., Masich, S., Goldie, K., Hoenger, A.

    Exp Cell Res 313(10), 2217-27 (2007)

    PubMed

    43

    Dissecting the 3-D structure of vimentin intermediate filaments by cryo-electron tomography

    Goldie, K., Wedig, T., Mitra, A. K., Aebi, U., Herrmann, H., Hoenger, A.

    J Struct Biol 158(3), 378-85 (2007)

    PubMed

    42

    Epidemiologic characteristics of catastrophic musculoskeletal injuries in thoroughbred racehorses

    Cruz, A. M., Poljak, Z., Filejski, C., Lowerison, M. L., Goldie, K., Martin, S. W., Hurtig, M. B.

    Am J Vet Res 68(12), 1370-5 (2007)

    PubMed

    41

    Visualization of cell microtubules in their native state

    Bouchet-Marquis, C., Zuber, B., Glynn, A. M., Eltsov, M., Grabenbauer, M., Goldie, K., Thomas, D., Frangakis, A. S., Dubochet, J., Chretien, D.

    Biol Cell 99(1), 45-53 (2007)

    PubMed

    40

    Structural rearrangements in tubulin following microtubule formation

    Krebs, A., Goldie, K., Hoenger, A.

    EMBO Rep 6(3), 227-32 (2005)

    PubMed

    39

    Surface-decoration of microtubules by human tau

    Santarella, R. A., Skiniotis, G., Goldie, K., Tittmann, P., Gross, H., Mandelkow, E. M., Mandelkow, E., Hoenger, A.

    J Mol Biol 339(3), 539-53 (2004)

    PubMed

    37

    Actin binding of a minispectrin

    Raae, A. J., Banuelos, S., Ylanne, J., Olausson, T., Goldie, K., Wendt, T., Hoenger, A., Saraste, M.

    Biochim Biophys Acta 1646(42006), 67-76 (2003)

    PubMed

    36

    Dimerization properties of a Xenopus laevis kinesin-II carboxy-terminal stalk fragment

    De Marco, V., De Marco, A., Goldie, K., Correia, J. J., Hoenger, A.

    EMBO Rep 4(7), 717-22 (2003)

    PubMed

    35

    Pure F-actin networks are distorted and branched by steps in the critical-point drying method

    Resch, G. P., Goldie, K., Hoenger, A., Small, J. V.

    J Struct Biol 137(3), 305-12 (2002)

    PubMed

    34

    Amyloid fibril formation by human stefin B in vitro: immunogold labelling and comparison to stefin A

    Zerovnik, E., Zavasnik-Bergant, V., Kopitar-Jerala, N., Pompe-Novak, M., Skarabot, M., Goldie, K., Ravnikar, M., Musevic, I., Turk, V.

    Biol Chem 383(5), 859-63 (2002)

    PubMed

    33

    De novo designed peptide-based amyloid fibrils

    Lopez De La Paz, M., Goldie, K., Zurdo, J., Lacroix, E., Dobson, C. M., Hoenger, A., Serrano, L.

    Proc Natl Acad Sci U S A 99(25), 16052-7 (2002)

    PubMed

    32

    Visualisation of the actin cytoskeleton by cryo-electron microscopy

    Resch, G. P., Goldie, K., Krebs, A., Hoenger, A., Small, J. V.

    J Cell Sci 115(Pt 9), 1877-82 (2002)

    PubMed

    31

    Microscopic evidence for a minus-end-directed power stroke in the kinesin motor ncd

    Wendt, T. G., Volkmann, N., Skiniotis, G., Goldie, K., Muller, J., Mandelkow, E., Hoenger, A.

    EMBO J 21(22), 5969-78 (2002)

    PubMed

    30

    Modulation of antigenicity related to changes in antibody flexibility upon lyophilization

    Taschner, N., Müller, S., Alumella, V. R., Goldie, K., Drake, A. F., Aebi, U., Arvinte, T.

    J Mol Biol 310(1), 169-79 (2001)

    PubMed

    29

    Op18/stathmin caps a kinked protofilament-like tubulin tetramer

    Steinmetz, M. O., Kammerer, R. A., Jahnke, W., Goldie, K., Lustig, A., van Oostrum, J.

    EMBO J 19(4), 572-80 (2000)

    PubMed

    28

    A new look at the microtubule binding patterns of dimeric kinesins

    Hoenger, A., Thormahlen, M., Diaz-Avalos, R., Doerhoefer, M., Goldie, K., Muller, J., Mandelkow, E.

    J Mol Biol 297(5), 1087-103 (2000)

    PubMed

    27

    Amyloid fibril formation from full-length and fragments of amylin

    Goldsbury, C., Goldie, K., Pellaud, J., Seelig, J., Frey, P., Müller, S., Kistler, J., Cooper, G. J., Aebi, U.

    J Struct Biol 130(42038), 352-62 (2000)

    PubMed

    26

    A distinct membrane current in rat lens fiber cells isolated under calcium-free conditions

    Eckert, R., Donaldson, P., Goldie, K., Kistler, J.

    Invest Ophthalmol Vis Sci 39(7), 1280-5 (1998)

    PubMed

    24

    Evaluating atomic models of F-actin with an undecagold-tagged phalloidin derivative

    Steinmetz, M. O., Stoffler, D., Müller, S., Jahn, W., Wolpensinger, B., Goldie, K., Engel, A., Faulstich, H., Aebi, U.

    J Mol Biol 276(1), 42010 (1998)

    PubMed

    22

    A correlative analysis of actin filament assembly, structure, and dynamics

    Steinmetz, M. O., Goldie, K., Aebi, U.

    J Cell Biol 138(3), 559-74 (1997)

    PubMed

    21

    Polymorphic fibrillar assembly of human amylin

    Goldsbury, C. S., Cooper, G. J., Goldie, K., Müller, S., Saafi, E. L., Gruijters, W. T., Misur, M. P., Engel, A., Aebi, U., Kistler, J.

    J Struct Biol 119(1), 17-27 (1997)

    PubMed

    20

    Structural studies on the 2.25-MDa homomultimeric phosphoenolpyruvate synthase from Staphylothermus marinus

    Harauz, G., Cicicopol, C., Hegerl, R., Cejka, Z., Goldie, K., Santarius, U., Engel, A., Baumeister, W.

    J Struct Biol 116(2), 290-301 (1996)

    PubMed

    19

    Structure and assembly properties of the intermediate filament protein vimentin: the role of its head, rod and tail domains

    Herrmann, H., Haener, Markus, Brettel, Monika, Müller, S., Goldie, K., Fedtke, Bettina, Lustig, Ariel, Franke, Werner W., Aebi, U.

    J Mol Biol 264(5), 933-953 (1996)

    PubMed

    18

    Functional significance of symmetrical versus asymmetrical GroEL-GroES chaperonin complexes

    Engel, A., Hayer-Hartl, M. K., Goldie, K., Pfeifer, G., Hegerl, R., Müller, S., da Silva, A. C., Baumeister, W., Hartl, F. U.

    Science 269(5225), 832-6 (1995)

    PubMed

    17

    Reconstitution of native-type noncrystalline lens fiber gap junctions from isolated hemichannels

    Kistler, J., Goldie, K., Donaldson, P., Engel, A.

    J Cell Biol 126(4), 1047-58 (1994)

    PubMed

    15

    Exploring native nuclear-pore complex structure and conformation by scanning force microscopy in physiological buffers

    Goldie, K., Pante, N., Engel, A., Aebi, U.

    Journal of Vacuum Science & Technology B 12(3), 1482-1485 (1994)

    DOI: DOI

    14

    Topology of the morphological domains of the chaperonin GroEL visualized by immuno-electron microscopy

    Martin, J., Goldie, K., Engel, A., Hartl, F. U.

    Biol Chem Hoppe Seyler 375(9), 635-9 (1994)

    PubMed

    13

    Towards atomic interpretation of F-actin filament three-dimensional reconstructions

    Bremer, A., Henn, C., Goldie, K., Engel, A., Smith, P. R., Aebi, U.

    J Mol Biol 242(5), 683-700 (1994)

    PubMed

    12

    Scan speed limit in atomic force microscopy

    Butt, H. -J., Siedle, P., Seifert, K., Fendler, K., Seege, T., Bamberg, E., Weisenhor, A. L., Goldie, K., Engel, A.

    J Microsc 169(1), 75-84 (1993)

    DOI: DOI

    11

    Ambient-pressure scanning probe microscopy of 2D regular protein arrays

    Schabert, F., Hefti, A., Goldie, K., Stemmer, A., Engel, A., Meyer, E., Overney, R., Guntherodt, H. J.

    Ultramicroscopy 42, 1118-1124 (1992)

    DOI: DOI

    9

    Factors influencing the precision of quantitative scanning transmission electron microscopy

    Müller, S., Goldie, K., Bürki, R., Häring, R., Engel, A.

    Ultramicroscopy 46(1-4), 317-334 (1992)

    DOI: DOI

    7

    Aspects of shell formation in eggs of the Tuatara, Sphenodon-Punctatus

    Packard, M. J., Thompson, M. B., Goldie, K., Vos, M.

    Journal of Morphology 197(2), 147-157 (1988)

    DOI: DOI

    5

    Classifying moths with the aid of SEM

    Gibbs, G. W., Goldie, K.

    New Zealand J Science 26(4), 553-553 (1983)

    4

    Electron-microscopic investigation of pollen in the trimeniaceae (Laurales)

    Sampson, F. B., Endress, P. K., Goldie, K.

    New Zealand J Science 26(4), 556-556 (1983)

    3

    Electron-microscopy in a centralized service facility

    Thomson, R., Goldie, K., Chalcraft, A., Abbott, D.

    New Zealand J Science 26(4), 557-558 (1983)

    2

    Facility Manager

    73218

    C-CINA, Biozentrum, Uni Basel, Mattenstrasse 26, Room P.34, CH-4058 Basel, Switzerland

    k.goldie@unibas.ch