Mohamed Chami

Current Project

BioEm Lab

    Short Biography

    Mohamed Chami from Marocco has been senior scientist in the Stahlberg group, CINA since 2009. He is specialized in high-resolution electron microscopy. His Post Doc experience focused on the crystallization of membrane proteins and was gained at the Biozentrum, University of Basel, Switzerland and the Institut Curie, Paris, France.

    Publications

    -+

    Self-assembly of a designed nucleoprotein architecture through multimodal interactions

    Subramanian, R.H., Smith, S.J., Alberstein, R.G., Bailey, J.B., Zhang, L., Cardone, G., Suominen, L., Chami, M., Stahlberg, H., Baker, T.S., Tezcan, F.A.

    ACS Central Science 4(11), 1578-1586 (2018)

    PubMed

    DOI: DOI

    99

    Structural basis for regulation of human acetyl-CoA carboxylase

    Hunkeler, M., Hagmann, A., Stuttfeld, E., Chami, M., Guri, Y., Stahlberg, H., Maier, T.

    Nature 558, 470-474 (2018)

    PubMed

    DOI: DOI

    97

    Cryo-EM analysis of homodimeric full-length LRRK2 and LRRK1 protein complexes

    Sejwal, K., Chami, M., Remigy, H., Vancraenenbroeck, R., Sibran, W., Sütterlin, R., Baumgartner, P., McLeod, R., Chartier-Harlin. M.-C., Baekelandt, V., Stahlberg, H., Taymans, J.-M.

    Scientific Reports 7(1), 8667-8678 (2017)

    PubMed

    DOI: DOI

    92

    Direct visualization of glutamate transporter elevator mechanism by high-speed AFM

    Ruan Y, Miyagi A, Wang X, Chami, M., Boudker O, Scheuring S,

    Proc Natl Acad Sci USA 114(7), 1584-1588 (2017)

    PubMed

    DOI: 10.1073/pnas.1616413114

    91

    Prepore Stability Controls Productive Folding of the BAM-independent Multimeric Outer Membrane Secretin PulD.

    Guilvout I., Brier S., Chami, M., Hourdel V., Francetic O., Pugsley A.P., Chamot-Rooke J., Huysmans G.H.

    J. Biol. Chem. 292(1), 328-338 (2017)

    PubMed

    DOI: 10.1074/jbc.M116.759498

    90

    Evidence for a LOS and a capsular polysaccharide in Capnocytophaga canimorsus.

    Renzi F, Ittig S, Sadovskaya I, Hess E, Lauber F, Dol M, Shin H, Mally M, Fiechter C, Sauder U, Chami, M., Cornelis G

    Sci Rep. 6(Article number: 38914), (2016)

    PubMed

    DOI: 10.1038/srep38914

    89

    Assembling of the Mycobacterium tuberculosis Cell Wall Core.

    Grzegorzewicz AE, de Sousa-d'Auria C, McNeil MR, Huc-Claustre E, Jones V, Petit C, Angala SK, Zemanová J, Wang Q, Belardinelli JM, Gao Q, Ishizaki Y, Mikušová K, Brennan PJ, Ronning DR, Chami, M., Houssin C, Jackson M.

    J Biol Chem. 291(26), 18867-79 (2016)

    DOI: 10.1074/jbc.M116.739227

    86

    Two-dimensional crystallization of the mouse serotonin 5-HT3A receptor

    Rheinberger, J., Hassaine, G., Chami, M., Stahlberg, H., Vogel, H., Li, X.

    Micron 90, 19-24 (2017)

    PubMed

    DOI: j.micron.2016.10.004

    85

    Investigation of the thin film crystallization of a DNA copolymer hybrid composed of chitosan.

    Ilyès Safir, Chami, M., Thomas Buergi, Corinne Nardin

    Polymer International 65(10), 1165-1171 (2016)

    DOI: 10.1002/pi.5165

    83

    Chaperonin–Dendrimer Conjugates for siRNA Delivery

    Martin G. Nussbaumer, Jason T. Duskey, Martin Rother, Kasper Renggli, Chami, M., Nico Bruns

    Advanced Science 133(12), 4476-4483 (2016)

    PubMed

    DOI: 10.1002/advs.201600046

    81

    Structural basis for haem piracy from host haemopexin by Haemophilus influenzae.

    Zambolin S., Clantin B., Chami, M., Hoos S., Haouz A., Villeret V., Delepelaire P.

    Nature Comm. 7(11590), (2016)

    PubMed

    DOI: 10.1038/ncomms11590

    80

    The lipidome associated with the gamma-secretase complex is required for its integrity and activity.

    Ayciriex, S., Gerber, H., Osuna, G.M., Chami, M., Stahlberg, H., Shevchenko, A., Fraering, P.C.

    Biochemical Journal 473(3), 321-334 (2016)

    PubMed

    DOI: 10.1042/BJ20150448

    78

    Glasslike Membrane Protein Diffusion in a Crowded Membrane.

    Munguira I., Casuso I., Takahashi H., Rico F., Miyagi A., Chami, M., Scheuring S.

    ACS Nano 10(2), 2584-90 (2016)

    PubMed

    DOI: 10.1021/acsnano.5b07595

    77

    Structure and assembly of the mouse ASC inflammasome by combined NMR spectroscopy and cryo-electron microscopy

    Sborgi, L., Ravotti, F., Dandey, V. P., Dick, M. S., Mazur, A., Reckel, S., Chami, M., Scherer, S., Huber, M., Bockmann, A., Egelman, E. H., Stahlberg, H., Broz, P., Meier, B. H., Hiller, S.

    Proc Natl Acad Sci U S A 112(43), 13237-42 (2015)

    PubMed

    76

    Diaminopimelic acid amidation in corynebacteriales: new insights into the role of LtsA in peptidoglycan modification

    Levefaudes, M., Patin, D., de Sousa-d'Auria, C., Chami, M., Blanot, D., Herve, M., Arthur, M., Houssin, C., Mengin-Lecreulx, D.

    J Biol Chem 290(21), 13079-94 (2015)

    PubMed

    75

    Lipids assist the membrane insertion of a BAM-independent outer membrane protein

    Huysmans, G. H., Guilvout, I., Chami, M., Nickerson, N. N., Pugsley, A. P.

    Sci Rep 5, 15068 (2015)

    PubMed

    74

    3D cryo-electron reconstruction of BmrA, a bacterial multidrug ABC transporter in an inward-facing conformation and in a lipidic environment

    Fribourg, P. F., Chami, M., Sorzano, C. O., Gubellini, F., Marabini, R., Marco, S., Jault, J. M., Levy, D.

    J Mol Biol 426(10), 2059-69 (2014)

    PubMed

    72

    pH-responsive PDMS-b-PDMAEMA micelles for intracellular anticancer drug delivery

    Car, A., Baumann, P., Duskey, J. T., Chami, M., Bruns, N., Meier, W.

    Biomacromolecules 15(9), 3235-45 (2014)

    PubMed

    71

    Bacterial secretins form constitutively open pores akin to general porins

    Disconzi, E., Guilvout, I., Chami, M., Masi, M., Huysmans, G. H., Pugsley, A. P., Bayan, N.

    J Bacteriol 196(1), 121-8 (2014)

    PubMed

    70

    Cryo-electron microscopy of membrane proteins

    Goldie, K., Abeyrathne, P., Kebbel, F., Chami, M., Ringler, P., Stahlberg, H.

    Methods Mol Biol 1117, 325-41 (2014)

    PubMed

    69

    The amine content of PEGylated chitosan Bombyx mori nanoparticles acts as a trigger for protein delivery

    Vasquez, D., Milusheva, R., Baumann, P., Constantin, D., Chami, M., Palivan, C. G.

    Langmuir 30(4), 965-75 (2014)

    PubMed

    68

    Independent domain assembly in a trapped folding intermediate of multimeric outer membrane secretins

    Guilvout, I., Chami, M., Disconzi, E., Bayan, N., Pugsley, A. P., Huysmans, G. H.

    Structure 22(4), 582-9 (2014)

    PubMed

    67

    Insights into bacteriophage T5 structure from analysis of its morphogenesis genes and protein components

    Zivanovic, Y., Confalonieri, F., Ponchon, L., Lurz, R., Chami, M., Flayhan, A., Renouard, M., Huet, A., Decottignies, P., Davidson, A. R., Breyton, C., Boulanger, P.

    J Virol 88(2), 1162-74 (2014)

    PubMed

    66

    Label-free, optical sensing of the supramolecular assembly into fibrils of a ditryptophan-DNA hybrid

    Gour, N., Abraham, J. N., Chami, M., Castillo, A., Verma, S., Vebert-Nardin, C.

    Chem Commun (Camb) 50(52), 6863-5 (2014)

    PubMed

    65

    Ligand-induced structural changes in the cyclic nucleotide-modulated potassium channel MloK1

    Kowal, J., Chami, M., Baumgartner, P., Arheit, M., Chiu, P. L., Rangl, M., Scheuring, S., Schroder, G. F., Nimigean, C. M., Stahlberg, H.

    Nat Commun 5, 3106 (2014)

    PubMed

    64

    Molecular basis for the fold organization and sarcomeric targeting of the muscle atrogin MuRF1

    Franke, B., Gasch, A., Rodriguez, D., Chami, M., Khan, M. M., Rudolf, R., Bibby, J., Hanashima, A., Bogomolovas, J., von Castelmur, E., Rigden, D. J., Uson, I., Labeit, S., Mayans, O.

    Open Biol 4, 130172 (2014)

    PubMed

    63

    Assessing the conformational changes of pb5, the receptor-binding protein of phage T5, upon binding to its Escherichia coli receptor FhuA

    Breyton, C., Flayhan, A., Gabel, F., Lethier, M., Durand, G., Boulanger, P., Chami, M., Ebel, C.

    J Biol Chem 288(42), 30763-72 (2013)

    PubMed

    62

    Molecular assembly of the aerolysin pore reveals a swirling membrane-insertion mechanism

    Degiacomi, M. T., Iacovache, I., Pernot, L., Chami, M., Kudryashev, M., Stahlberg, H., van der Goot, F. G., Dal Peraro, M.

    Nat Chem Biol 9(10), 623-9 (2013)

    PubMed

    60

    Electron microscopy analysis of 2D crystals of membrane proteins

    Abeyrathne, P.D., Arheit, M., Kebbel, F., Castano-Diez, D., Goldie, K., Chami, M., Renault, L., Kühlbrandt, W., Stahlberg, H.

    Comprehensive Biophysics 1, 277-310 (2012)

    DOI: 10.1016/B978-0-12-374920-8.00119-3

    59

    A single amino acid substitution changes the self-assembly status of a type IV piliation secretin

    Nickerson, N. N., Abby, S. S., Rocha, E. P., Chami, M., Pugsley, A. P.

    J Bacteriol 194(18), 4951-8 (2012)

    PubMed

    58

    Biochemical disclosure of the mycolate outer membrane of Corynebacterium glutamicum

    Marchand, C. H., Salmeron, C., Bou Raad, R., Meniche, X., Chami, M., Masi, M., Blanot, D., Daffe, M., Tropis, M., Huc, E., Le Marechal, P., Decottignies, P., Bayan, N.

    J Bacteriol 194(3), 587-97 (2012)

    PubMed

    57

    Characterization of the motion of membrane proteins using high-speed atomic force microscopy

    Casuso, I., Khao, J., Chami, M., Paul-Gilloteaux, P., Husain, M., Duneau, J. P., Stahlberg, H., Sturgis, J. N., Scheuring, S.

    Nat Nanotechnol 7(8), 525-9 (2012)

    PubMed

    56

    CO2 permeability of cell membranes is regulated by membrane cholesterol and protein gas channels

    Itel, F., Al-Samir, S., Oberg, F., Chami, M., Kumar, M., Supuran, C. T., Deen, P. M., Meier, W., Hedfalk, K., Gros, G., Endeward, V.

    FASEB J 26(12), 5182-91 (2012)

    PubMed

    55

    Directed self-assembly of lipid nanotubes from inverted hexagonal structures

    Sugihara, K., Chami, M., Derenyi, I., Voros, J., Zambelli, T.

    ACS Nano 6(8), 6626-32 (2012)

    PubMed

    54

    Outer membrane targeting of Pseudomonas aeruginosa proteins shows variable dependence on the components of Bam and Lol machineries

    Hoang, H. H., Nickerson, N. N., Lee, V. T., Kazimirova, A., Chami, M., Pugsley, A. P., Lory, S.

    MBio 2(6), (2011)

    PubMed

    53

    Assembly of a protein "brush" by end-grafting titin fragments to liposomes

    Chami, M., Franke, B., Mayans, O., Kreplak, L.

    J Biosci Bioeng 112(2), 178-9 (2011)

    PubMed

    52

    Automated screening of 2D crystallization trials using transmission electron microscopy: a high-throughput tool-chain for sample preparation and microscopic analysis

    Coudray, N., Hermann, G., Caujolle-Bert, D., D., Karathanou, A., Erne-Brand, F., Buessler, J. L., Daum, P., Plitzko, J. M., Chami, M., Müller, U., Kihl, H., Urban, J. P., Engel, A., Remigy, H. W.

    J Struct Biol 173(2), 365-74 (2011)

    PubMed

    51

    Biocompatible functionalization of polymersome surfaces: a new approach to surface immobilization and cell targeting using polymersomes

    Egli, S., Nussbaumer, M. G., Balasubramanian, V., Chami, M., Bruns, N., Palivan, C., Meier, W.

    J Am Chem Soc 133(12), 4476-83 (2011)

    PubMed

    49

    Coupled cell-free synthesis and lipid vesicle insertion of a functional oligomeric channel MscL MscL does not need the insertase YidC for insertion in vitro

    Berrier, C., Guilvout, I., Bayan, N., Park, K. H., Mesneau, A., Chami, M., Pugsley, A. P., Ghazi, A.

    Biochim Biophys Acta 1808(1), 41-6 (2011)

    PubMed

    48

    Efficient topical delivery of plasmid DNA to lung in vivo mediated by putative triggered, PEGylated pDNA nanoparticles

    Aissaoui, A., Chami, M., Hussein, M., Miller, A. D.

    J Control Release 154(3), 275-84 (2011)

    PubMed

    47

    Multimerization-defective variants of dodecameric secretin PulD

    Guilvout, I., Nickerson, N. N., Chami, M., Pugsley, A. P.

    Res Microbiol 162(2), 180-90 (2011)

    PubMed

    46

    A deficiency in arabinogalactan biosynthesis affects Corynebacterium glutamicum mycolate outer membrane stability

    Bou Raad, R., Meniche, X., de Sousa-d'Auria, C., Chami, M., Salmeron, C., Tropis, M., Labarre, C., Daffe, M., Houssin, C., Bayan, N.

    J Bacteriol 192(11), 2691-700 (2010)

    PubMed

    45

    Structural characterization of the rod cGMP phosphodiesterase 6

    Goc, A., Chami, M., Lodowski, D. T., Bosshart, P., Moiseenkova-Bell, V., Baehr, W., Engel, A., Palczewski, K.

    J Mol Biol 401(3), 363-73 (2010)

    PubMed

    43

    The 2DX robot: a membrane protein 2D crystallization Swiss Army knife

    Iacovache, I., Biasini, M., Kowal, J., Kukulski, W., Chami, M., van der Goot, F. G., Engel, A., Remigy, H. W.

    J Struct Biol 169(3), 370-8 (2010)

    PubMed

    42

    Functional reconstitution into liposomes of purified human RhCG ammonia channel

    Mouro-Chanteloup, I., Cochet, S., Chami, M., Genetet, S., Zidi-Yahiaoui, N., Engel, A., Colin, Y., Bertrand, O., Ripoche, P.

    PLoS One 5(1), e8921 (2010)

    PubMed

    41

    Isolation and characterization of low nucleic acid (LNA)-content bacteria

    Wang, Y., Hammes, F., Boon, N., Chami, M., Egli, T.

    ISME J 3(8), 889-902 (2009)

    PubMed

    40

    Phage T5 straight tail fiber is a multifunctional protein acting as a tape measure and carrying fusogenic and muralytic activities

    Boulanger, P., Jacquot, P., Plancon, L., Chami, M., Engel, A., Parquet, C., Herbeuval, C., Letellier, L.

    J Biol Chem 283(20), 13556-64 (2008)

    PubMed

    39

    Projection structure of a member of the amino acid/polyamine/organocation transporter superfamily

    Casagrande, F., Ratera, M., Schenk, A. D., Chami, M., Valencia, E., Lopez, J. M., Torrents, D., Engel, A., Palacin, M., Fotiadis, D.

    J Biol Chem 283(48), 33240-8 (2008)

    PubMed

    38

    Artificial binding proteins (Affitins) as probes for conformational changes in secretin PulD

    Krehenbrink, M., Chami, M., Guilvout, I., Alzari, P. M., Pecorari, F., Pugsley, A. P.

    J Mol Biol 383(5), 1058-68 (2008)

    PubMed

    37

    Structural properties of AMP-activated protein kinase: dimerization, molecular shape, and changes upon ligand binding

    Riek, U., Scholz, R., Konarev, P., Rufer, A., Suter, M., Nazabal, A., Ringler, P., Chami, M., Müller, S., Neumann, D., Forstner, M., Hennig, M., Zenobi, R., Engel, A., Svergun, D., Schlattner, U., Wallimann, T.

    J Biol Chem 283(26), 18331-43 (2008)

    PubMed

    36

    Conductive supports for combined AFM-SECM on biological membranes

    Frederix, P. L., Bosshart, P. D., Akiyama, T., Chami, M., Gullo, M. R., Blackstock, J. J., Dooleweerdt, K., de Rooij, N. F., Staufer, U., Engel, A.

    Nanotechnology 19(38), 384004 (2008)

    PubMed

    35

    Direct visualization of the outer membrane of mycobacteria and corynebacteria in their native state

    Zuber, B., Chami, M., Houssin, C., Dubochet, J., Griffiths, G., Daffe, M.

    J Bacteriol 190(16), 5672-80 (2008)

    PubMed

    34

    Vacuolar protein sorting: two different functional states of the AAA-ATPase Vps4p

    Hartmann, C., Chami, M., Zachariae, U., de Groot, B. L., Engel, A., Grütter, M. G.

    J Mol Biol 377(2), 352-63 (2008)

    PubMed

    33

    In vitro multimerization and membrane insertion of bacterial outer membrane secretin PulD

    Guilvout, I., Chami, M., Berrier, C., Ghazi, A., Engel, A., Pugsley, A. P., Bayan, N.

    J Mol Biol 382(1), 13-23 (2008)

    PubMed

    32

    Projection maps of three members of the KdgM outer membrane protein family

    Signorell, G. A., Chami, M., Condemine, G., Schenk, A. D., Philippsen, A., Engel, A., Remigy, H. W.

    J Struct Biol 160(3), 395-403 (2007)

    PubMed

    31

    Reprint of "Structural and mechanistic aspects of Amt/Rh proteins" [J. Struct. Biol. 158 (2007) 472-481]

    Javelle, A., Lupo, D., Li, X. D., Merrick, M., Chami, M., Ripoche, P., Winkler, F. K.

    J Struct Biol 159(2), 243-52 (2007)

    PubMed

    30

    Self-organization behavior of methacrylate-based amphiphilic di- and triblock copolymers

    Rakhmatullina, E., Braun, T., Chami, M., Malinova, V., Meier, W.

    Langmuir 23(24), 12371-9 (2007)

    PubMed

    29

    Structural and mechanistic aspects of Amt/Rh proteins

    Javelle, A., Lupo, D., Li, X. D., Merrick, M., Chami, M., Ripoche, P., Winkler, F. K.

    J Struct Biol 158(3), 472-81 (2007)

    PubMed

    28

    YaeT-independent multimerization and outer membrane association of secretin PulD

    Collin, S., Guilvout, I., Chami, M., Pugsley, A. P.

    Mol Microbiol 64(5), 1350-7 (2007)

    PubMed

    27

    Bacterial outer membrane secretin PulD assembles and inserts into the inner membrane in the absence of its pilotin

    Guilvout, I., Chami, M., Engel, A., Pugsley, A. P., Bayan, N.

    EMBO J 25(22), 5241-9 (2006)

    PubMed

    26

    Cytotoxin ClyA from Escherichia coli assembles to a 13-meric pore independent of its redox-state

    Eifler, N., Vetsch, M., Gregorini, M., Ringler, P., Chami, M., Philippsen, A., Fritz, A., Müller, S., Glockshuber, R., Engel, A., Grauschopf, U.

    EMBO J 25(11), 2652-61 (2006)

    PubMed

    25

    Double hexameric ring assembly of the type III protein translocase ATPase HrcN

    Müller, S., Pozidis, C., Stone, R., Meesters, C., Chami, M., Engel, A., Economou, A., Stahlberg, H.

    Mol Microbiol 61(1), 119-25 (2006)

    PubMed

    24

    Reconstitution of two recombinant LSm protein complexes reveals aspects of their architecture, assembly, and function

    Zaric, B., Chami, M., Remigy, H., Engel, A., Ballmer-Hofer, K., Winkler, F. K., Kambach, C.

    J Biol Chem 280(16), 16066-75 (2005)

    PubMed

    23

    Structural insights into the secretin PulD and its trypsin-resistant core

    Chami, M., Guilvout, I., Gregorini, M., Remigy, H. W., Müller, S., Valerio, M., Engel, A., Pugsley, A. P., Bayan, N.

    J Biol Chem 280(45), 37732-41 (2005)

    PubMed

    22

    The crucial role of trehalose and structurally related oligosaccharides in the biosynthesis and transfer of mycolic acids in Corynebacterineae

    Tropis, M., Meniche, X., Wolf, A., Gebhardt, H., Strelkov, S., Chami, M., Schomburg, D., Kramer, R., Morbach, S., Daffe, M.

    J Biol Chem 280(28), 26573-85 (2005)

    PubMed

    21

    Functional and structural characterization of the minimal Sec translocase of the hyperthermophile Thermotoga maritime

    Pretz, M. G., Remigy, H., Swaving, J., Albers, S. V., Garrido, V. G., Chami, M., Engel, A., Driessen, A. J.

    Extremophiles 9(4), 307-16 (2005)

    PubMed

    20

    Trimeric structure of OprN and OprM efflux proteins from Pseudomonas aeruginosa, by 2D electron crystallography

    Lambert, O., Benabdelhak, H., Chami, M., Jouan, L., Nouaille, E., Ducruix, A., Brisson, A.

    J Struct Biol 150(1), 50-7 (2005)

    PubMed

    19

    A polyketide synthase catalyzes the last condensation step of mycolic acid biosynthesis in mycobacteria and related organisms

    Portevin, D., De Sousa-D'Auria, C., Houssin, C., Grimaldi, C., Chami, M., Daffe, M., Guilhot, C.

    Proc Natl Acad Sci U S A 101(1), 314-9 (2004)

    PubMed

    18

    Importance of mycoloyltransferases on the physiology of Corynebacterium glutamicum

    Kacem, R., De Sousa-D'Auria, C., Tropis, M., Chami, M., Gounon, P., Leblon, G., Houssin, C., Daffe, M.

    Microbiology 150(Pt 1), 73-84 (2004)

    PubMed

    17

    Micelles of lipid-oligonucleotide conjugates: implications for membrane anchoring and base pairing

    Gosse, C., Boutorine, A., Aujard, I., Chami, M., Kononov, A., Cogne-Laage, E., Allemand, J. F., Li, J., Jullien, L.

    J Phys Chem B 108(20), 6485-97 (2004)

    PubMed

    16

    Membrane protein reconstitution and crystallization by controlled dilution

    Remigy, H. W., Caujolle-Bert, D., D., Suda, K., Schenk, A., Chami, M., Engel, A.

    FEBS Lett 555(1), 160-9 (2003)

    PubMed

    15

    Structural analysis and evidence for dynamic emergence of Bacillus anthracis S-layer networks

    Couture-Tosi, E., Delacroix, H., Mignot, T., Mesnage, S., Chami, M., Fouet, A., Mosser, G.

    J Bacteriol 184(23), 6448-56 (2002)

    PubMed

    13

    Characterization of a hyperthermophilic P-type ATPase from Methanococcus jannaschii expressed in yeast

    Morsomme, P., Chami, M., Marco, S., Nader, J., Ketchum, K. A., Goffeau, A., Rigaud, J. L.

    J Biol Chem 277(33), 29608-16 (2002)

    PubMed

    12

    Charting and unzipping the surface layer of Corynebacterium glutamicum with the atomic force microscope

    Scheuring, S., Stahlberg, H., Chami, M., Houssin, C., Rigaud, J. L., Engel, A.

    Mol Microbiol 44(3), 675-84 (2002)

    PubMed

    11

    Three-dimensional structure by cryo-electron microscopy of YvcC, an homodimeric ATP-binding cassette transporter from Bacillus subtilis

    Chami, M., Steinfels, E., Orelle, C., Jault, J. M., Di Pietro, A., Rigaud, J. L., Marco, S.

    J Mol Biol 315(5), 1075-85 (2002)

    PubMed

    10

    Structure of the cell envelope of corynebacteria: importance of the non-covalently bound lipids in the formation of the cell wall permeability barrier and fracture plane

    Puech, V., Chami, M., Lemassu, A., Laneelle, M. A., Schiffler, B., Gounon, P., Bayan, N., Benz, R., Daffe, M.

    Microbiology 147(Pt 5), 1365-82 (2001)

    PubMed

    9

    Two-dimensional crystallization of membrane proteins: the lipid layer strategy

    Levy, D., Chami, M., Rigaud, J. L.

    FEBS Lett 504(3), 187-93 (2001)

    PubMed

    8

    Use of octyl beta-thioglucopyranoside in two-dimensional crystallization of membrane proteins

    Chami, M., Pehau-Arnaudet, G., Lambert, O., Ranck, J. L., Levy, D., Rigaud, J. L.

    J Struct Biol 133(1), 64-74 (2001)

    PubMed

    7

    Use of detergents in two-dimensional crystallization of membrane proteins

    Rigaud, J., Chami, M., Lambert, O., Levy, D., Ranck, J.

    Biochim Biophys Acta 1508(42006), 112-28 (2000)

    PubMed

    6

    S-layer protein production by Corynebacterium strains is dependent on the carbon source

    Soual-Hoebeke, E., de Sousa-D'Auria, C., Chami, M., Baucher, M. F., Guyonvarch, A., Bayan, N., Salim, K., Leblon, G.

    Microbiology 145 ( Pt 12), 3399-408 (1999)

    PubMed

    5

    The S-layer protein of Corynebacterium glutamicum is anchored to the cell wall by its C-terminal hydrophobic domain

    Chami, M., Bayan, N., Peyret, J. L., Gulik-Krzywicki, T., Leblon, G., Shechter, E.

    Mol Microbiol 23(3), 483-92 (1997)

    PubMed

    3

    Molecular biology of S-layers

    Bahl, H., Scholz, H., Bayan, N., Chami, M., Leblon, G., Gulik-Krzywicki, T., Shechter, E., Fouet, A., Mesnage, S., Tosi-Couture, E., Gounon, P., Mock, M., Conway de Macario, E., Macario, A. J., Fernandez-Herrero, L. A., Olabarria, G., Berenguer, J., Blaser, M. J., Kuen, B., Lubitz

    FEMS Microbiol Rev 20(42006), 47-98 (1997)

    PubMed

    2

    Organization of the outer layers of the cell envelope of Corynebacterium glutamicum: a combined freeze-etch electron microscopy and biochemical study

    Chami, M., Bayan, N., Dedieu, J., Leblon, G., Shechter, E., Gulik-Krzywicki, T.

    Biol Cell 83(42038), 219-229 (1995)

    PubMed

    1

    Head BioEM Lab

    +41 61 387 32 24

    C-CINA, Biozentrum, Uni Basel, Mattenstrasse 26, Room P30, CH-4058 Basel, Switzerland

    Mohamed.Chami@unibas.ch