Philippe Ringler

Current Project

Structural analysis of proteins by transmission electron microscopy (TEM). In particular, TEM analysis of antibody constructs and conjugates, epitope mapping, conformation and homogeneity analysis.

Publications

-+

Cryo-EM structure of alpha-synuclein fibrils

Guerrero-Ferreira, R., Taylor, N., Mona, D., Ringler, P., Lauer, M.E., Riek, R., Britschgi, M., Stahlberg, H.

eLife 7(e36402), (2018)

PubMed

DOI: DOI

43

Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene

Seuring, C., Ayyer, Filippaki, Barthelmess, Longchamp, Ringler, P., Pardini, Wojtas, Coleman, Dörner, Fuglerud, Hammarin, Habenstrein, Langkilde, Loquet, Meents, Riek, Stahlberg, H., Boutet, Hunter, Koglin, Liang, Ginn, Millane, Frank, Barty, Chapman

Nature Communications 9(1), 1836 (2018)

PubMed

DOI: DOI

42

Amyloid Fibril Polymorphism: Almost Identical on the Atomic Level, Mesoscopically Very Different

Seuring, C., Verasdonck, J., Ringler, P., Cadalbert, R., Stahlberg, H., Böckmann,A., Meier, B.H., Riek, R.

J. Phys. Chem. 121(8), 1783-1792 (2017)

PubMed

DOI: 10.1021/acs.jpcb.6b10624

41

Characterization of mAb dimers reveals predominant dimer forms common in therapeutic mAbs

Plath, F., Ringler, P., Graff, A., Stahlberg, H., Lauer, M., Rufer, A., Graewert, M., Svergun, D., Kellermann, G., Winkler, C., Stücke, J.O., Koulov, A., Schnaible, V.

mAbs 8(5), 928-940 (2016)

PubMed

DOI:

39

Principles governing the self-assembly of coiled-coil protein nanoparticles

Indelicato, G., Wahome, N., Ringler, P., Müller, S., Nieh, M.-P., Burkhard, P., Twarock, R.

Biophys J 110(3), 646-660 (2016)

PubMed

DOI: 10.1016/j.bpj.2015.10.057

38

Cholesteryl ester transfer between lipoproteins does not require the formation of a ternary tunnel complex with the cholesteryl ester transfer protein

Lauer, M.E., Graff, A., Rufer, A.C., Maugeais, C., von der Mark, E., Matile, H., D'Arcy, B., Magg, C., Ringler, P., Müller, S., Scherer, S., Dernick, G., Thoma, R., Hennig, M., Niesor, E.J., Stahlberg, H.

J. Struct. Biol. 194(2), 191-198 (2016)

DOI: 10.1016/j.jsb.2016.02.016

37

Optimizing the design of protein nanoparticles as carriers for vaccine applications

Doll, T. A., Neef, T., Duong, N., Lanar, D. E., Ringler, P., Müller, S., Burkhard, P.

Nanomedicine 11(7), 1705-13 (2015)

PubMed

35

openBEB: open biological experiment browser for correlative measurements

Ramakrishnan, C., Bieri, A., Sauter, N., Roizard, S., Ringler, P., Müller, S., Goldie, K., Enimanev, K., Stahlberg, H., Rinn, B., Braun, T.

BMC Bioinformatics 15, 84 (2014)

PubMed

33

Structural and mechanistic paradigm of leptin receptor activation revealed by complexes with wild-type and antagonist leptins

Moharana, K., Zabeau, L., Peelman, F., Ringler, P., Stahlberg, H., Tavernier, J., Savvides, S. N.

Structure 22(6), 866-77 (2014)

PubMed

32

Review: Cryo-electron microscopy of membrane proteins

Goldie, K., Abeyrathne, P., Kebbel, F., Chami, M., Ringler, P., Stahlberg, H.

In: Kuo J. (eds) Electron Microscopy. Methods in Molecular Biology (Methods and Protocols) Vol. 1117(Humana Press, Totowa, NJ, USA), 325-341 (2014)

PubMed

DOI: DOI

31

The rhodopsin-transducin complex houses two distinct rhodopsin molecules

Jastrzebska, B., Ringler, P., Palczewski, K., Engel, A.

J Struct Biol 182(2), 164-72 (2013)

PubMed

29

Connecting micro-fluidics to electron microscopy

Kemmerling, Simon, Ziegler, J., Schweighauser, G., Arnold, S., Giss, D., Müller, S., Ringler, P., Goldie, K., Goedecke, N., Hierlemann, A., Stahlberg, H., Engel, A., Braun, T.

J Struct Biol 177(1), 128-134 (2012)

PubMed

27

Rhodopsin-transducin heteropentamer: three-dimensional structure and biochemical characterization

Jastrzebska, B., Ringler, P., Lodowski, D. T., Moiseenkova-Bell, V., Golczak, M., Müller, S., Palczewski, K., Engel, A.

J Struct Biol 176(3), 387-94 (2011)

PubMed

26

Direct control of the spatial arrangement of gold nanoparticles in organic-inorganic hybrid superstructures

Hermes, J. P., Sander, F., Peterle, T., Cioffi, C., Ringler, P., Pfohl, T., Mayor, M.

Small 7(7), 920-9 (2011)

PubMed

25

Bipartite design of a self-fibrillating protein copolymer with nanopatterned peptide display capabilities

Bruning, M., Kreplak, L., Leopoldseder, S., Müller, S., Ringler, P., Duchesne, L., Fernig, D. G., Engel, A., Ucurum-Fotiadis, Z., Mayans, O.

Nano Lett 10(11), 4533-7 (2010)

PubMed

24

Molecular architecture of Streptococcus pneumoniae TIGR4 pili

Hilleringmann, M., Ringler, P., Müller, S., De Angelis, G., Rappuoli, R., Ferlenghi, I., Engel, A.

EMBO J 28(24), 3921-30 (2009)

PubMed

22

Omp85(Tt) from Thermus thermopiles HB27: an ancestral type of the Omp85 protein family

Nesper, J., Brosig, A., Ringler, P., Patel, G. J., Müller, S., Kleinschmidt, J. H., Boos, W., Diederichs, K., Welte, W.

J Bacteriol 190(13), 4568-75 (2008)

PubMed

21

Structural properties of AMP-activated protein kinase: dimerization, molecular shape, and changes upon ligand binding

Riek, U., Scholz, R., Konarev, P., Rufer, A., Suter, M., Nazabal, A., Ringler, P., Chami, M., Müller, S., Neumann, D., Forstner, M., Hennig, M., Zenobi, R., Engel, A., Svergun, D., Schlattner, U., Wallimann, T.

J Biol Chem 283(26), 18331-43 (2008)

PubMed

20

Cytotoxin ClyA from Escherichia coli assembles to a 13-meric pore independent of its redox-state

Eifler, N., Vetsch, M., Gregorini, M., Ringler, P., Chami, M., Philippsen, A., Fritz, A., Müller, S., Glockshuber, R., Engel, A., Grauschopf, U.

EMBO J 25(11), 2652-61 (2006)

PubMed

19

Immunogenicity and structural characterisation of an in vitro folded meningococcal siderophore receptor (FrpB, FetA)

Kortekaas, J., Müller, S., Ringler, P., Gregorini, M., Weynants, . E., Rutten, l., Bos, Martine P., Tommassen, J.

Microbes and Infection 8(8), 2145-2153 (2006)

PubMed

18

Impact of disease mutations on the desmin filament assembly process

Bar, H., Mucke, N., Ringler, P., Müller, S., Kreplak, L., Katus, H. A., Aebi, U., Herrmann, H.

J Mol Biol 360(5), 1031-42 (2006)

PubMed

17

Molecular anatomy of a trafficking organelle

Takamori, S., Holt, M., Stenius, K., Lemke, E. A., Gronborg, M., Riedel, D., Urlaub, H., Schenck, S, Bruegger, B., Ringler, P., Müller, S., Rammner, B., Graeter, F., Hub, J. S., De Groot, B. L., Mieskes, G., Moriyama, Y.

Cell 127(4), 831-846 (2006)

PubMed

DOI: DOI

16

The V-antigen of Yersinia forms a distinct structure at the tip of injectisome needles

Müller, C. A., Broz, P., Müller, S., Ringler, P., Erne-Brand, F., Sorg, I., Kuhn, M., Engel, A., Cornelis, G. R.

Science 310(5748), 674-6 (2005)

PubMed

15

Molecular architecture and assembly mechanism of Drosophila tripeptidyl peptidase II

Rockel, B., Peters, J., Müller, S., Seyit, G., Ringler, P., Hegerl, R., Glaeser, R. M., Baumeister, W.

Proc Natl Acad Sci U S A 102(29), 10135-40 (2005)

PubMed

14

Structure and assembly of the pseudopilin PulG

Köhler, R., Schaefer, K., Müller, S., Vignon, G., Diederichs, K., Philippsen, A., Ringler, P., Pugsley, A. P., Engel, A., Welte, W.

Molec Microbiol 54(3), 647-664 (2004)

PubMed

13

Structure and electrophysiological properties of the YscC secretin from the type III secretion system of Yersinia enterocolitica

Burghout, P., van Boxtel, R., Van Gelder, P., Ringler, P., Müller, S., Tommassen, J., Koster, M.

J Bacteriol 186(14), 4645-54 (2004)

PubMed

12

Self-assembly of proteins into designed networks

Ringler, P., Schulz, G. E.

Science 302(5642), 106-9 (2003)

PubMed

11

The aquaporin sidedness revisited

Scheuring, S., Tittmann, P., Stahlberg, H., Ringler, P., Borgnia, M., Agre, P., Gross, H., Engel, A.

J. Mol. Biol. 299(5), 1271-1278 (2000)

PubMed

9

Structure of the water channel AqpZ from Escherichia coli revealed by electron crystallography

Ringler, P., Borgnia, M. J., Stahlberg, H., Maloney, P. C., Agre, P., Engel, A.

J Mol Biol 291(5), 1181-90 (1999)

PubMed

8

Two-dimensional crystallization of Escherichia coli lactose permease

Zhuang, J., Prive, G. G., Werner, G. E., Ringler, P., Kaback, H. R., Engel, A.

J Struct Biol 125(1), 63-75 (1999)

PubMed

7

High resolution AFM topographs of the Escherichia coli water channel aquaporin Z

Scheuring, S., Ringler, P., Borgnia, M., Stahlberg, H., Muller, D. J., Agre, P., Engel, A.

EMBO J 18(18), 4981-7 (1999)

PubMed

6

Imaging streptavidin 2D crystals on biotinylated lipid monolayers at high resolution with the atomic force microscope

Scheuring, S., Muller, D. J., Ringler, P., Heymann, J. B., Engel, A.

J Microsc 193(1), 28-35 (1999)

PubMed

5

Synthesis of an acetylcholine receptor-specific toxin derivative regioselectively labeled with an undecagold cluster

Kessler, P., Kotzyba-Hibert, F., Leonetti, M., Bouet, F., Ringler, P., Brisson, A., Menez, A., Goeldner, M. P., Hirth, C.

Bioconjug Chem 5(3), 199-204 (1994)

PubMed

2

Two-dimensional crystallization of proteins on planar lipid films and structure determination by electron crystallography

Brisson, A., Olofsson, A., Ringler, P., Schmutz, M., Stoylova, S.

Biol Cell 80(42038), 221-8 (1994)

PubMed

1

Senior Scientist

+41 61 387 32 14

C-CINA, Biozentrum, Uni Basel, Mattenstrasse 26, Room P20, CH-4058 Basel, Switzerland

philippe.ringler@unibas.ch