Cryo-Sectioning (CEMOVIS)

Applications: To obtain 3D structural information for samples that are too thick for cryo-electron microscopy and cryo-electron tomography. Resolution ~1nm. 

Method: Tissues or cells are cryo-protected and vitrified at high pressure. Slices are cut from the blocks using a cryo-ultramicrotome and imaged at cryo temperatures by transmission electron microscopy.

Advantages:  Samples are imaged in a close-to-native environment. Reconstruction of the image series recorded from sequential slices gives the 3D structure.

Disadvantages: Freezing and cutting artifacts can occur. Resolution in the z direction is limited by the thickness of the slice.

Sample requirements: Please contact us.