Thin Sectioning

Application: To determine the ultrastructure of tissues and cells at 2nm resolution by transmission electron microscopy (TEM).

Method: Tissues or cells are chemically fixed, dehydrated, embedded in resin and stained. Thin slices are cut from the resin block using an ultramicrotome and imaged at room temperature by TEM. Immuno-EM is possible before the embedding or after the sectioning steps.

Advantages: Higher resolution than CEMOVIS. Immuno-EM can be used to locate specific proteins.

Disadvantages: The sample is treated chemically and dehydrated, which can lead to artifacts.

Sample requirements: Please contact us.