FOCUS is a software system that enables high-throughput, online processing of cryo-EM data. Typically, FOCUS is used next to an automated cryo-EM instrument.
FOCUS is open source. It can be found on FOCUS-EM.org.
FOCUS is written in C++, uses Qt libraries, and interacts with a large number of other programs through scripts in C-shell or python. Third-party programs can be MotionCor2, gCTF, CTFFIND4, the MRC package, or IMOD, etc.. Various scripts exist to implement different workflows.
Scripts for single particle movie processing, for electron tomography processing, or for 2D crystal image processing are provided.